Procedures for Quality Control of RNA Samples for Use in Quantitative Reverse Transcription PCR
نویسندگان
چکیده
The quality of any scientific data is directly proportional to that of the original starting samples, or simply ‘garbage in, garbage out’. In most circumstances it is logical to work with the highest quality material possible. However, for some experiments the highest quality possible is still a serious compromise from perfection. The degree to which the standard of input material influences final quantitative reverse transcription polymerase chain reaction (qRT-PCR) data and, potentially, the resulting scientific conclusion, is outlined in this chapter.
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